Entrepreneurship is a source of financial, economic and social prosperity for countries as well as their regions. The objective of this article was to identify whether the young generation is interested in entrepreneurship, especially students of economics at public universities, and whether there are regional cross-border disparities in this respect (i.e., comparison of regional differences between neighbouring countries). 540 students of economics at public universities based in border areas were included in the survey group. The selected universities were the Faculty of Social Sciences in Ústí nad Labem (Czech Republic) and the Hochschule für Technik und Wirtschaft Dresden (Germany). Written interviews were selected as the research method. German students were established to be statistically significantly more interested in entrepreneurship. The second objective was to verify whether there are differences in the personality traits of students who are willing to set up their own business from those who are not. The personality traits tested representation of traits, such as independence, risk tolerance, creativity, initiative and responsibility. It was established using the chi-squared test, Saphiro-Wilk test, t-test, test for two binomial distributions and the Kruskal-Wallis test that different traits are not exhibited by students according to the region or country in which they live and study, but according to the criterion of whether they want to set up their own business or not. Accordingly, there are no differences between Czech and German students in the monitored traits but there are statistically significant differences between the traits of students who want to set up their own business and those who do not want to - both between German and Czech students.